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Research pages: GPCRs Ligand-Gated Ion Channels Techology Development Animal Models

The DDC has a strong background in the development of new screening technology for early drug discovery. The Biomolecular Analysis (Irth) group focuses on the development of novel analytical technologies that simultaneously measure chemical (concentration, molecular mass, absorption spectra) and biochemical parameters (affinity for receptors) of analytes. This integration of chemical analysis and biomolecular screening allows the discovery of bioactive compounds in complex mixtures. Applications of the novel techniques developed are targeted mainly towards drug discovery and early ADME research.


Selected publications

AR de Boer, T Letzel, DA van Elswijk, H Lingeman, WMA Niessen and H Irth (2004) On-line coupling of high-performance liquid chromatography to a continuous-flow enzyme assay based on electrospray ionization mass spectrometry. Anal Chem. 76:3155-3161

RJE Derks, AC Hogenboom, G van der Zwan and H Irth (2003) On-line continuous-flow, multi-protein biochemical assays for the characterization of bioaffinity compounds using electrospray quadrupole time-of-flight mass spectrometry. Anal Chem. 75:3376-3384

J Hirata, CF de Jong, MM van Dongen, J Buijs, F Ariese, H Irth and C Gooijer (2004) A flow injection kinase assay system based on time-resolved fluorescence resonance energy-transfer detection in the millisecond range. Anal Chem. 76:4292-4298

AC Hogenboom, AR de Boer, RJE Derks and H Irth (2001) Continuous-flow, on-line monitoring of biospecific interactions using electrospray mass spectrometry. Anal Chem. 73:3816-3823

JG Krabbe, H Lingeman, WMA Niessen and H Irth (2003) Ligand-exchange detection of phosphorylated peptides using liquid chromatography electrospray mass spectrometry. Anal Chem. 75:6853-6860

A Multi-protein high-throughput screening assay technology based on mass spectrometry was developed by the Biomolecular analysis group. The method allows the screening of multiple proteins and provides simultaneously information on the biochemical interactions and the chemical structure of bioactive compounds. A world-wide patent application has been filed in 2000 to protect the intellectual property rights for this method.