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Facilities
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The DDC has state-of-the art analytical equipment for the development
of new high content screening assays. The group has access to
various systems for LC-MS (Micro-, Nano-LC-MS) and GC-MS. Moreover,
for proteomic approaches the group has invested recently a lot in MS
infrastructure. Currently available are a 4700 Proteomics Discovery
System, 2 QTOFs, 2 Ion-traps and a high-resolution MS.
The DDC has various laboratories dedicated to Medicinal
Chemistry. Equipment for parallel synthesis/purification (Flashmaster
automated chromatography system, Shimadzu preparative LC-MS) is well in
place. On one hand the group is using microwave-assisted chemistry to
speed up the synthesis process, whereas a variety of computer-assisted
approaches are included in the discovery programs in order to improve
the quality of the synthesized molecules.
The DDC is fully equipped for standard molecular biology work, cell
biology, radioligand binding studies, neurotransmitter release studies
and a variety of signal transduction assays. Laboratories are operated
with state of the art equipment, including:
- QPCR (ABI) 7900 with robotics
- Zeiss laser scanning confocal microscope,
- Guava personal FACS 96-well,
- Tecan pipetting robots,
- BMG-Novostar multilabel reader,
- Perkin Elmer Victor2 multilabel reader,
- Wallac Microbeta multilabel reader.
The DDC has ample experience with a large number of fully validated in vivo models in rodents, including:
- Operant drug/sucrose self-administration
- 5-choice serial reaction time task
- Delayed-reward task
- Elevated plus maze
- Vogel conflict test
- Automated video tracking for psychomotor activity
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